Re: ORGLIST:P estimation

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From: Jacek Achremowicz (mfachrem$##$cyf-kr.edu.pl)
Date: Fri Jun 25 2004 - 05:34:15 EDT


There is a fairly simple method of the estimation of PO4 in starch.
1. Satrch must be hydrolized (DP <=3) (using acid or enzymes)
2. cleave PO4 groups using acid phospatase (from Asp. orhyzae or other)
3. Estimate the PO4 content with one of the spectrophotometric methods.
I used modified Fiske-Subbarow-Samotus method: PO4 + (NH4)2MoO4 at pH <1
formgives a complex which after reduction with metgives blue solution
with lambda max at 660 nm.
The method is very sensitive and precise and reproducible.
You must plot standard curve each time before analysis.

Good luck!

Jacek Achremowicz
Pharmacognosy Department
Jagiellonian University
Cracow
Poland

On Tue, 15 Jun 2004, rajkn wrote:

> Hi:
> I would greatly appreciate
> information on the estimation of
> P in starch/polysaccahrides as PO4.
> Thanks in advance.
>
> KNRajasekharan
> Chemistry
> Univ Kerala
> India
>
>
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