Date view Thread view Subject view Author view Attachment view

From: Jacob Zabicky (zabicky$##$
Date: Fri Mar 12 2004 - 20:42:24 EST

Thanks also to Helmer Södergård, Christoph D. Hahn and K. N.
Rajasekharan, for their useful replies (excerpts below).

All the best,



Reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) or its
diethyl derivative has been applied widely to assess lipid peroxidation
in biological material (1). The reaction yields a red MDA-TBA adduct,
the product of 2 mol of TBA plus 1 mol of MDA (2). The colored complex
can be quantified spectrophotometrically from its visible absorbance
(max 532 nm) (3)(4) or by spectrofluorometry (exc 532 nm, em 553 nm)
(5)(6) and is readily extractable into organic solvents such as butanol
(7). Preanalytical factors aside (8)(9)(10), interfering chromogens
include several other ordinary side-products of lipid autooxidation,
alkanals, alkenals, and alkadienals as well as bile pigments, cyclic
peroxides, carbohydrates, and amino acids
(11)(12)(13)(14)(15)(16)(17)(18).   ...

Ref 2 =

Nair V, Turner GA. The thiobarbituric acid test for lipid peroxidation:
structure of the adduct with malondialdehyde. Lipids 1984;19:804-805.

Ref 3 = Sinnhuber RO, Yu TC, Chang Yu T. Characterization of the red
pigment formed in the 2-thiobarbituric acid determination of oxidative
rancidity. Food Res 1958;23:626-634.

Look at

then point on "equations"!

Best regards


In Fig. 1 of the attached paper, there's a structure that could help. I
found this by searching the ACS journals on for
terms "malondialdehyde" and "thiobarbituric" anywhere in article.





I think this is the
  structure...Can't give  any ref
supporting it offhand...
It is a cyanine type dye....

ORGLIST - Organic Chemistry Mailing List
Website / Archive / FAQ:
To post a message (TO EVERYBODY) send to everybody$##$
To unsubscribe, send to everybody-request$##$ the message: unsubscribe your_orglist_password your_address
List coordinator: Joao Aires de Sousa (coordinator$##$

Date view Thread view Subject view Author view Attachment view

This archive was generated by hypermail 2.1.4 : Sun Mar 14 2004 - 11:37:01 EST