Re: ORGLIST:MDA + TBA

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From: Jacob Zabicky (zabicky$##$bgumail.bgu.ac.il)
Date: Fri Mar 12 2004 - 20:42:24 EST


Thanks also to Helmer Södergård, Christoph D. Hahn and K. N.
Rajasekharan, for their useful replies (excerpts below).

All the best,

Jacob

-------------

Reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) or its
diethyl derivative has been applied widely to assess lipid peroxidation
in biological material (1). The reaction yields a red MDA-TBA adduct,
the product of 2 mol of TBA plus 1 mol of MDA (2). The colored complex
can be quantified spectrophotometrically from its visible absorbance
(max 532 nm) (3)(4) or by spectrofluorometry (exc 532 nm, em 553 nm)
(5)(6) and is readily extractable into organic solvents such as butanol
(7). Preanalytical factors aside (8)(9)(10), interfering chromogens
include several other ordinary side-products of lipid autooxidation,
alkanals, alkenals, and alkadienals as well as bile pigments, cyclic
peroxides, carbohydrates, and amino acids
(11)(12)(13)(14)(15)(16)(17)(18).   ...

Ref 2 =

Nair V, Turner GA. The thiobarbituric acid test for lipid peroxidation:
structure of the adduct with malondialdehyde. Lipids 1984;19:804-805.

Ref 3 = Sinnhuber RO, Yu TC, Chang Yu T. Characterization of the red
pigment formed in the 2-thiobarbituric acid determination of oxidative
rancidity. Food Res 1958;23:626-634.

Look at

   
http://life.nthu.edu.tw/~rrandd/89s1/b871614/frame/summer/antioxidant/
lipid.html

then point on "equations"!

Best regards
helmer

--------------------------

In Fig. 1 of the attached paper, there's a structure that could help. I
found this by searching the ACS journals on http://pubs.acs.org for
terms "malondialdehyde" and "thiobarbituric" anywhere in article.

Regards,
Christoph

---------------------------

 

  



I think this is the
  structure...Can't give  any ref
supporting it offhand...
It is a cyanine type dye....
Rajasekharan
 
 

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